Heller, Richard 1954-

Flow cytometry protocols by Mark J Jaroszeski( )

16 editions published between 1997 and 1998 in English and held by 1,026 WorldCat member libraries worldwide

In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology

Electrochemotherapy, electrogenetherapy, and transdermal drug delivery : electrically mediated delivery of molecules to cells by Mark J Jaroszeski( )

15 editions published between 2000 and 2010 in English and held by 327 WorldCat member libraries worldwide

Reviews all aspects of the delivery of therapeutic molecules to cells using electrical impulse. Surveys basic physical principles that govern cell permeabilization by pulsed electric fields, describes instrumentation and electrodes, and summarizes preclinical and clinical trial results. Focuses on drug, gene, and transdermal delivery techniques, providing detailed examples of drug delivery using electric fields from a variety of pulse generators and electrodes

Bioelectrics by Hidenori Akiyama( )

15 editions published between 2016 and 2018 in English and held by 243 WorldCat member libraries worldwide

This book focuses on bioelectrics, a new multidisciplinary field encompassing engineering and biology with applications to the medical, environmental, food, energy, and biotechnological fields. At present, 15 universities and institutes in Japan, the USA and the EU comprise the International Consortium of Bioelectrics, intended to advance this novel and important research field. This book will serve as an introductory resource for young scientists and also as a textbook for use by both undergraduate and graduate students - the world's first such work solely devoted to bioelectrics

Electroporation protocols : preclinical and clinical gene medicine by Shulin Li( )

7 editions published in 2014 in English and held by 185 WorldCat member libraries worldwide

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field. --

Flow Cytometry Protocols by Mark J Jaroszeski( )

1 edition published in 1998 in English and held by 6 WorldCat member libraries worldwide

In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology

Flow cytometry protocols by Mark J Jaroszeski( Book )

2 editions published between 1997 and 1998 in English and held by 5 WorldCat member libraries worldwide

Electrically mediated delivery of molecules to cells( Book )

1 edition published in 2000 in English and held by 2 WorldCat member libraries worldwide

Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery : Electrically Mediated Delivery of Molecules to Cells. Methods in Molecular Medicine, Volume 37( )

2 editions published in 2000 in English and held by 2 WorldCat member libraries worldwide

Leading experts comprehensively review all aspects of the delivery of therapeutic molecules to cells using electrical impulses - i.e., the extraordinarily promising new areas of electrogenetherapy, electrochemotherapy, and transdermal drug delivery. Their survey ranges from outlines of the basic physical principles that govern cell permeabilization by pulsed electric fields, to descriptions of the current state-of-the-art in instrumentation and electrodes, to summaries of preclinical and clinical trial results. The authors focus on drug, gene, and transdermal delivery techniques, providing detailed examples of drug delivery using electric fields from a variety of pulse generators and electrodes. Comprehensive and authoritative, Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells provides entre to an immensely practical set of in vivo electroporation techniques, including electrogenetherapy, electrochemotherapy, and transdermal drug delivery - techniques holding great promise for all those working toward better therapies for cancer, metabolic diseases, and vaccination

7 Arabesken für zwei Flöten und Klavier, (2000/2001) : : by Richard Heller( )

1 edition published in 2022 in Undetermined and held by 1 WorldCat member library worldwide

Electroporation Protocols : Preclinical and Clinical Gene Medicine by Shulin Li( )

1 edition published in 2014 in English and held by 1 WorldCat member library worldwide

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture, and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues, and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field